Features of CD3 +- and CD3+CD4 +-peripheral blood cells at different pregnancy stages in women with anamnese infertility

Infertile marriage is a complex socio-economic problem; the frequency of infertility in Ukraine 17-20%, which prompts further study its mechanisms, including immune ones, order to develop appropriate methods correction. Purpose - characterize phenotypic features T-cells and their subpopulation T-helpers (CD3+CD4+) with expression intracellular cytokines women history at different stages pregnancy. Materials methods. Relative levels immunocompetent cells (cl) blood, CD3+CD4+cells, 436 non-pregnant (the reference group n) 514 pregnant first trimester second pregnancy were determined using cellular cytofluorimeter corresponding test systems. Characteristics indicators terms groups analyzed: 4-7 weeks (140 women); b 8-9 (163 c 10-12 (133 d 13-18 (63 e 19-28 (15 women) compared n among themselves. Results. The 12 are characterized by high blood (CD3+CD4+-cl), activation according markers CD25 HLA-DR, for 18 secretion pro-inflammatory IFN-γ + TNF-α, anti-inflammatory IL-4 was observed throughout 27 follow-up. percentage CD3+CD4+IFN-γ+- CD3+CD4+TNF-α+-L highest during weeks, from 13th week these 20th do not differ ones. Since beginning pregnancy, relative number low IL-10 has significantly decreased; within such increased group, most vulnerable due possible weak reaction link state tolerance. Conclusions. characteristics peripheral system cell CD phenotypes cytokine indicate participation mechanisms individual analysis allows negative predictions improvement course infertility. research carried out accordance principles Helsinki Declaration. protocol approved Local Ethics Committee participating institution. informed consent patient obtained conducting studies. No conflict interests declared authors. Keywords: CD-phenotype cells, cytokines, women,